Analysis of circulating tumor and cancer stem cells provides new opportunities in diagnosis and treatment of small cell lung cancer

Skurikhin, E. G., Ermakova, N., Zhukova, M., Pershina, O., Pan, E., Pakhomova, A., Kogai, L., Goldberg, V., Simolina, E., Skurikhina, V., Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X, Kubatiev, A., Morozov, S. G., Kushlinskii, N. and Dygai, A. (2022) Analysis of circulating tumor and cancer stem cells provides new opportunities in diagnosis and treatment of small cell lung cancer. International Journal of Molecular Sciences, 23 (18). 10853. ISSN 1422-0067 doi: 10.3390/ijms231810853

Abstract/Summary

Current methods for diagnosis and treatment of small cell lung cancer (SCLC) have only modest efficacy. In this pilot study, we analysed circulating tumour cells (CTCs) and cancer stem cells (CSCs) in patients with SCLC to search for new diagnostic and prognostic markers and novel approaches to improve the treatment of the disease. In other forms of lung cancer, we have shown a heterogeneity of blood CTCs and CSCs populations, as well as changes in other cell populations (ALDH+, CD87+CD276+ and EGF+Axl‒) in smokers. A number of CTCs and CSCs in patients with SCLC have been shown to be resistant to chemotherapy (CT). High cytotoxic activity and resistance to apoptosis of reprogrammed CD3+CD8+ T-lymphocytes (rTcells) in relation to naive CD3+CD8+ T- lymphocytes was demonstrated in a smoking patient with SCLC (Patient G) in vitro. The target for rTcells was patient G's blood CSCs. Reprogramming of CD3+CD8+ T-lymphocytes was carried out with the MEK1/2 inhibitor and PD-1/PD-L1 pathway blocker nivolumab. The training procedure was performed with a suspension of dead CTCs and CSCs obtained from patient`s G blood. The presented data show a new avenue for personalized SCLC diagnosis and targeted improvement of chemotherapy based on the use of both CTCs and CSCs.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/107307
Identification Number/DOI	10.3390/ijms231810853
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	MDPI
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