Method for the production of cotton somatic embryos. United States Patent Application

Syngenta Biotechnology Inc (2003) Method for the production of cotton somatic embryos. United States Patent Application. US20030143744. doi: US20030143744

Abstract/Summary

The present invention provides Inter alia, a method for the production of cotton somatic embryos comprising (a) isolating a totipotent stomatal cell-containing epidermal explant from leaf material excised from a cotton plant; and (b) culturing said explant in a basal medium which comprises an embryogenic callus-inducing quantity of an auxin and a cytokinin under an embryogenic callus inducing intensity of light until embryogenic callus is formed; and (c) sub-culturing said embryogenic callus onto a somatic embryo differentiation media to produce said somatic embryos. Plants may be regenerated from the somatic embryos and in a particular embodiment of the invention said totipotent stomatal cell is transformed, prior to the inducement of embryogenic callus, with a polynucleotide that provides for a desired agronomic trait.

Item Type	Patent
URI	https://reading-clone.eprints-hosting.org/id/eprint/10724
Identification Number/DOI	US20030143744
Divisions	Life Sciences > School of Biological Sciences
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