From food to mobility: investigating a screening assay for new automotive antioxidants using the stable radical DPPH

Higgins, C. L., Filip, S. V., Afsar, A., Colquhoun, H. M. and Hayes, W. ORCID: https://orcid.org/0000-0003-0047-2991 (2021) From food to mobility: investigating a screening assay for new automotive antioxidants using the stable radical DPPH. ChemistrySelect, 6 (34). pp. 9179-9184. ISSN 2365-6549 doi: 10.1002/slct.202102472

Abstract/Summary

By taking inspiration from the food industry, an assay was investigated as a potential screening tool to test the efficiency of new phenolic antioxidants. The method was based on the spectrophotometric measurement of the stable free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) which, in its radical form, has an absorbance maxima at 515 nm. The disappearance of this absorbance band, upon reaction with an antioxidant, was monitored to reveal the kinetic pathway of the reaction, which was defined simply as either fast, medium or slow. Adaptation of the assay was attempted for application to biofuels and oil-based automotive fluids whereby the effect of polar and non-polar solvents on the kinetics of the reaction was investigated. In addition, the stoichiometry of the radical scavenging reaction was also analysed to give an insight into the structure-activity relationships of phenolic antioxidants.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/100065
Identification Number/DOI	10.1002/slct.202102472
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	Wiley
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