Impact of dairy fat manipulation on endothelial function and lipid regulation in human aortic endothelial cells exposed to human plasma samples: an in vitro investigation from the RESET study

Markey, O., Garcimartín, A., Vasilopoulou, D., Kliem, K. E. ORCID: https://orcid.org/0000-0002-0058-8225, Fagan, C. C. ORCID: https://orcid.org/0000-0002-2101-8694, Humphries, D. J., Todd, S. ORCID: https://orcid.org/0000-0002-9981-923X, Givens, D. I. ORCID: https://orcid.org/0000-0002-6754-6935, Lovegrove, J. A. ORCID: https://orcid.org/0000-0001-7633-9455 and Jackson, K. G. ORCID: https://orcid.org/0000-0002-0070-3203 (2023) Impact of dairy fat manipulation on endothelial function and lipid regulation in human aortic endothelial cells exposed to human plasma samples: an in vitro investigation from the RESET study. European Journal of Nutrition. ISSN 1436-6215 doi: 10.1007/s00394-023-03284-9

Abstract/Summary

Purpose Longer-term intake of fatty acid (FA)-modified dairy products (SFA-reduced, MUFA-enriched) was reported to attenuate postprandial endothelial function in humans, relative to conventional (control) dairy. Thus, we performed an in vitro study in human aortic endothelial cells (HAEC) to investigate mechanisms underlying the effects observed in vivo. Methods This sub-study was conducted within the framework of the RESET study, a 12-week randomised controlled crossover trial with FA-modified and control dairy diets. HAEC were incubated for 24 h with post-intervention plasma samples from eleven adults (age: 57.5±6.0 years; BMI: 25.7±2.7 kg/m2 ) at moderate cardiovascular disease risk following representative sequential mixed meals. Markers of endothelial function and lipid regulation were assessed. Results Relative to control, HAEC incubation with plasma following the FA-modified treatment increased postprandial NOx production (P-interaction=0.019), yet up-regulated relative E-selectin mRNA gene expression (P-interaction=0.011). There was no impact on other genes measured. Conclusion Incubation of HAEC with human plasma collected after longer-term dairy fat manipulation had a beneficial impact on postprandial NOx production. Further ex vivo research is needed to understand the impact of partial replacement of SFA with unsaturated fatty acids in dairy foods on pathways involved in endothelial function.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/114680
Identification Number/DOI	10.1007/s00394-023-03284-9
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR) Life Sciences > School of Agriculture, Policy and Development > Department of Animal Sciences Interdisciplinary Research Centres (IDRCs) > Institute for Food, Nutrition and Health (IFNH) Science > School of Mathematical, Physical and Computational Sciences > Department of Mathematics and Statistics Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
Publisher	Springer
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