PECAM-1 expression and activity negatively regulate multiple platelet signaling pathways

Jones, C. I. ORCID: https://orcid.org/0000-0001-7537-1509, Garner, S. F., Moraes, L. A., Kaiser, W. J., Rankin, A., Bloodomics Consortium, Ouwehand, W. H., Goodall, A. H. and Gibbins, J. M. ORCID: https://orcid.org/0000-0002-0372-5352 (2009) PECAM-1 expression and activity negatively regulate multiple platelet signaling pathways. FEBS Letters, 583 (22). pp. 3618-3624. ISSN 0014-5793 doi: 10.1016/j.febslet.2009.10.037

Abstract/Summary

Platelet endothelial cell adhesion molecule-1 (PECAM-1) inhibits platelet response to collagen and may also inhibit two other major platelet agonists ADP and thrombin although this has been less well explored. We hypothesized that the combined effect of inhibiting these three platelet activating pathways may act to significantly inhibit thrombus formation. We demonstrate a negative relationship between PECAM-1 surface expression and platelet response to cross-linked collagen related peptide (CRP-XL) and ADP, and an inhibitory effect of PECAM-1 clustering on platelet response to CRP-XL, ADP and thrombin. This combined inhibition of multiple signaling pathways results in a marked reduction in thrombus formation. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.