Assessment of antimicrobial activity, mode of action and volatile compounds of etlingera pavieana essential oil

Naksang, P. ORCID: https://orcid.org/0000-0002-9593-9381, Tongchitpakdee, S., Thumanu, K., Oruna-Concha, M. J. ORCID: https://orcid.org/0000-0001-7916-1592, Niranjan, K. ORCID: https://orcid.org/0000-0002-6525-1543 and Rachtanapun, C. (2020) Assessment of antimicrobial activity, mode of action and volatile compounds of etlingera pavieana essential oil. Molecules, 25 (14). 3245. ISSN 1420-3049 doi: 10.3390/molecules25143245

Abstract/Summary

Etlingera pavieana (Pierre ex Gagnep.) R.M.S. is a rhizomatous plant in the Zingiberaceae family which could be freshly eaten, used as a condiment or as a traditional remedy. Our work investigated the chemical composition and antimicrobial activity of the E. pavieana essential oils extracted from the rhizome (EOEP). We extracted the EOEP from the rhizome by hydrodistillation and analyzed the chemical composition by headspace solid-phase microextraction coupled with gas chromatography/mass spectrometry (HS-SPME-GC/MS). A total of 22 volatile compounds were identified where trans-anethole (78.54%) and estragole (19.36%) were the major components in the EOEP. The antimicrobial activity of EOEP was evaluated based on the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values using the broth dilution method and enumerating cell death overtime. Our work shows that the EOEP exhibits potent antibacterial activity against foodborne pathogenic Gram-positive bacteria, namely Bacillus cereus, Staphylococcus aureus and Listeria monocytogenes in the range of 0.1–0.3% (v/v). We further investigated the mechanism of EOEP inhibition using Synchrotron Fourier transformation infrared (FTIR) microspectroscopy. Here, we show significant differences in DNA/nucleic acid, proteins and cell membrane composition in the bacterial cell. To conclude, EOEP exhibited antimicrobial activity against foodborne pathogens, especially the Gram-positive bacteria associated with ready-to-eat (RTE) food and, thus, has the potential to serve as a natural preservative agent in RTE products.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/92241
Identification Number/DOI	10.3390/molecules25143245
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Research Group
Uncontrolled Keywords	Etlingera pavieana, antimicrobial activity, essential oil, foodborne pathogens, mode of action
Publisher	MDPI
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