Bioremediation potential of Cd by transgenic yeast expressing a metallothionein gene from Populus trichocarpa

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De Oliveira, V. H., Ullah, I. ORCID: https://orcid.org/0000-0002-9367-6741, Dunwell, J. M. ORCID: https://orcid.org/0000-0003-2147-665X and Tibbett, M. ORCID: https://orcid.org/0000-0003-0143-2190 (2020) Bioremediation potential of Cd by transgenic yeast expressing a metallothionein gene from Populus trichocarpa. Ecotoxicology and Environmental Safety, 202. 110917. ISSN 0147-6513 doi: 10.1016/j.ecoenv.2020.110917

Abstract/Summary

Cadmium (Cd) is an extremely toxic environmental pollutant with high mobility in soils, which can contaminate groundwater, increasing its risk of entering the food chain. Yeast biosorption can be a low-cost and effective method for removing Cd from contaminated aqueous solutions. We transformed wild-type Saccharomyces cerevisiae (WT) with two versions of a Populus trichocarpa gene (PtMT2b) coding for a metallothionein: one with the original sequence (PtMT2b ‘C’) and the other with a mutated sequence, with an amino acid substitution (C3Y, named here: PtMT2b ‘Y’). WT and both transformed yeasts were grown under Cd stress, in agar (0; 10; 20; 50 µM Cd) and liquid medium (0; 10; 20 µM Cd). Yeast growth was assessed visually and by spectrometry OD600. Cd removal from contaminated media and intracellular accumulation were also quantified. PtMT2b ‘Y’ was also inserted into mutant strains: fet3fet4, zrt1zrt2 and smf1, and grown under Fe-, Zn- and Mn-deficient media, respectively. Yeast strains had similar growth under 0 µM, but differed under 20 µM Cd, the order of tolerance was: WT < PtMT2b ‘C’ < PtMT2b ‘Y’, the latter presenting 37% higher growth than the strain with PtMT2b ‘C’. It also extracted ~80% of the Cd in solution, and had higher intracellular Cd than WT. Mutant yeasts carrying PtMT2b ‘Y’ had slightly higher growth in Mn- and Fe-deficient media than their non-transgenic counterparts, suggesting the transgenic protein may chelate these metals. S. cerevisiae carrying the altered poplar gene offers potential for bioremediation of Cd from wastewaters or other contaminated liquids.

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Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/91328
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Publisher	Elsevier
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Date Deposited:	23 Jun 2020 14:09	Date item deposited into CentAUR
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