Anther culture with different genotypes of opium poppy (Papaver somniferum L.): effect of cold treatment

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Dieu, P. and Dunwell, J. M. ORCID: https://orcid.org/0000-0003-2147-665X (1988) Anther culture with different genotypes of opium poppy (Papaver somniferum L.): effect of cold treatment. Plant Cell, Tissue and Organ Culture, 12 (3). pp. 263-271. ISSN 0167-6857 doi: 10.1007/BF00034367

Abstract/Summary

This study concerns anther culture and the production of microspore-derived calluses and plants of the opium poppy (Papaver somniferum L.). It was confirmed that growth regulators were necessary for microspore callus production. Cold treatment (7 d at 7 °C) of the buds prior to culture lead to a twofold increase in the frequency of responsive anthers and in the number of calluses per 100 anthers plated. Callus was produced from cultured anthers of several genotypes, covering a wide genetic background. Step by step removal of growth regulators from the culture medium promoted organogenesis and plant regeneration. Most regenerated plants were diploid. The overall process of microspore embryogenesis closely resembled that described in previous reports on somatic callus production and plant regeneration from poppy hypocotyls in vitro.

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Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/86984
Identification Number/DOI	10.1007/BF00034367
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Uncontrolled Keywords	Papaver somniferum, poppy, anther, pollen, haploid.
Publisher	Springer
Download/View statistics	View download statistics for this item

Deposit Details

Date Deposited:	19 Dec 2019 11:59	Date item deposited into CentAUR
Last Modified:	09 Jun 2024 04:17	Date item last modified

References

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