Anther culture of Lolium temulentum, Festuca pratensis and Lolium x Festuca Hybrids. II. Anther and pollen development in vivo and in vitro

Download

Full text not archived in this repository.

Advice

Please see our End User Agreement.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

Tools

Lists

Rose, J. B., Dunwell, J. M. ORCID: https://orcid.org/0000-0003-2147-665X and Sunderland, N. (1987) Anther culture of Lolium temulentum, Festuca pratensis and Lolium x Festuca Hybrids. II. Anther and pollen development in vivo and in vitro. Annals of Botany, 60 (2). pp. 203-214. ISSN 0305-7364 doi: 10.1093/oxfordjournals.aob.a087438

Abstract/Summary

The various pathways of pollen development were investigated in cultured anthers of Lolium temulentum, Festuca pratensis and the L. multiflorum x F. pratensis hybrid 'Elmet'. In all three, development from the vegetative cell was the predominant pathway of pollen callus development. However, there were characteristic differences in the behaviour of the generative cell. In L. temulentum it remained attached to the pollen wall and degenerated, whereas in F. pratensis it divided. In 'Elmet' it detached from the pollen wall and remained undivided. Both polarized and unpolarized partitioned calluses were observed. Development of the fusion product of the vegetative and generative nuclei were also observed in anthers of L. temulentum. Anomalous grains were not found to be major source of pollen calluses. Sections of anthers of L. temulentum were used to investigate the origin of S pollen grains, the small pale-staining grains which denote pollen dimorphism. Such grains form out of contact with the tapetum and are therefore determined before or during meiosis (i.e. before harvest of anthers for culture). Sections were also used to demonstrate the influence of the duration of pretreatment on the development of the middle layer of the anther wall.

Altmetric Badge

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/86982
Identification Number/DOI	10.1093/oxfordjournals.aob.a087438
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Uncontrolled Keywords	Festuca pratensis, Loliym temulentum, Lolium x Festuca, anther culture, haploid, microspore, pollen.
Publisher	Oxford University Press
Download/View statistics	View download statistics for this item

Deposit Details

Date Deposited:	19 Dec 2019 11:54	Date item deposited into CentAUR
Last Modified:	09 Jun 2024 01:27	Date item last modified

References

BENNETT, M.D., RAO, M.K., SMITH, J.B. and BAYLISS, M.W., 1973. Cell development in the anther, the ovule, and the young seed of Trilicum aestivum L. var. Chinese Spring. Philosophical Transactions of the Royal Society of London. B. Biological Sciences 266, 39-81. COLLINS, G.B., DUNWELL, J.M. and SUNDERLAND, N., 1974. Irregular microspore formation in Datura innoxia and its relevance to anther culture. Protoplasma 82, 365-78. DALE, P.J., 1975. Pollen dimorphism and anther culture of barley. Planta 127, 213-20. DUNWELL, J.M., 1985a. Anther and ovary culture, pp. 1-44 In Cereal Tissue and Cell Culture, eds S.W.J. Bright and M.G.K. Jones, 304 pp. Martinus Nijhoff, Dordrecht. DUNWELL, J.M., 1985b. Embryogenesis from pollen in vitro, pp. 49-76. In Biotechnology in Plant Science, eds M. Zaitlin, P. Day and A. Hollaender. Academic Press, New York. HEBERLE-BORS, E., 1985. In vitro haploid formation from pollen: a critical review. Theoretical and Applied Genetics 71, 361-374. HORNER, M. and STREET, H. E., 1978. Pollen dimorphism -origin and significance in pollen plant formation by anther culture. Annals of Botany 42, 763-71. IDZIKOWSKA, K., PONITKA, A. and MLODZIANOWSKI, F., 1982. Pollen dimorphism and androgenesis in Hordeum vulgare. Ada Societatis Botanicorum Poloniae 51, 153-6. PAN, J-L., GAO, G-H. and DAN, H., 1983. Initial types of wheat pollen cells and their development in anther culture, pp. 117-29. In Cell and Tissue Culture Techniques for Cereal Crop Improvement, 455 pp. Science Press, Beijing. QU, R-D. and CHEN, Y. 1984. Pathways of androgenesis and observations on cultured pollen grains in rice (Oryza saliva subsp. Keng). Ada Botanica Sinica 26, 580-7. ROSE, J.B., DUNWELL, J.M. and SUNDERLAND, N., 1986. Anther culture of Sorghum bicolor (L.) Moench. II. Pollen development in vivo and in vitro. Plant Cell, Tissue and Organ Culture 6, 23-31. ROSE, J.B., DUNWELL, J.M. and SUNDERLAND, N., 1987. Anther culture of Lolium temulentum, Festuca pratensis, and Lolium x Festuca hybrids. I. Influence of pretreatment, culture medium and culture incubation conditions on callus production and differentiation. Annals of Botany 60, 191-201 SUNDERLAND, N. and DUNWELL, J.M., 1977. Anther and pollen culture, pp. 223-65. In Plant Tissue and Cell Culture, ed. H. E. Street, 503 pp. 2nd edn. Blackwell Scientific Publications, Oxford. SUNDERLAND, N. and EVANS, L.J., 1980. Multicellular pollen formation in cultured barley anthers. II. The A, B and C pathways. Journal of Experimental Botany 31, 501-14. SUNDERLAND, N., ROBERTS, M., EVANS, L.J. and WILDON, D.C, 1979. Multicellular pollen formation in cultured barley anthers. I. Independent division of the generative and vegetative cells. Journal of Experimental Botany 30, 1133-44.

University Staff: Request a correction | Centaur Editors: Update this record

Search Google Scholar