Irregular microspore formation in Datura innoxia and its relevance to anther culture

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Collins, G. B., Dunwell, J. M. ORCID: https://orcid.org/0000-0003-2147-665X and Sunderland, N. (1974) Irregular microspore formation in Datura innoxia and its relevance to anther culture. Protoplasma, 82 (4). pp. 365-378. doi: 10.1007/BF01275729

Abstract/Summary

Meiotic irregularities in pollen mother cells of Datura innoxia lead to the formation of diploid (uninucleate) spores, and to multinucleate spores containing 2, 3 or 4 haploid nuclei. At the free microspore stage, irregular spores form three distinct size-classes having mean diameters of 46 (class II), 52 (III) and 64 (IV) ~tm, compared with 36 ~tm (I) for regular haploid spores. At the beginning of flowering, irregular spores account for about 0.8%(400-450) of the total pollen per anther, but the frequency increases with plant age, and towards the end of flowering, it may be as high as 8~ Irregular spores give rise either to non-haploid pollen grains (2 n, 3 n, 4 n) consisting of one generative and one vegetative nucleus, or to multinucleate pollen grains containing several generative and vegetative nuclei which may be of the same or of different ploidy. In cultured anthers, irregular spores or young pollen grains derived from them, participate in embryogenesis and give rise to non-haploid proembryoids. These are seen as contributing to the complement of non-haploid plants that ultimately emerge from the anthers. Such plants will be heterozygous in contrast to non-haploid plantlets generated from haploid pollen by nuclear fusion.

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Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/86969
Identification Number/DOI	10.1007/BF01275729
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Uncontrolled Keywords	Pollen Mother Cell, Pollen, Vegetative Nucleus
Publisher	Springer
Download/View statistics	View download statistics for this item

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Date Deposited:	19 Dec 2019 11:06	Date item deposited into CentAUR
Last Modified:	23 Jan 2024 18:13	Date item last modified

References

Belling, J., and A.F. Blakeslee, 1923: The reduction division in haploid, diploid, triploid and tetraploid Daturas. Proc. Nat. Acad. Sc. N.Y.9, 106–111. Bergner, A.D., J.L. Cartledge, and A.F. Blakeslee, 1934: Chromosome behaviour due to a gene which prevents metaphase pairing inDatura. Cytologia6, 19–37. Blakeslee, A.F., and A.G. Avery, 1934: Three genes located in the 21.22 chromosome of the Jimson weed. J. Hered.25, 395–404. Dowrick, G.J., 1957: The influence of temperature on meiosis. Heredity 11, 37–49. Engvild, K.C., I. Linde-Laursen, and A. Lundqvist, 1972: Anther cultures of Datura innoxia: flower bud stage and embryoid level of ploidy. Hereditas 72, 331–332. Geier, T., und H.W. Kohlenbach, 1973: Entwicklung von Embryonen und embryogenem Kallus aus Pollenkörnern von Datura meteloides und Datura innoxia. Protoplasma 78, 381–396. Guha, S., and S.C. Maheshwari, 1964: In vitro production of embryos from anthers of Datura. Nature (Lond.)204, 497. Höglund, M., 1970: Meiosis in Solanum phureja. Hereditas 66, 183–188. Iyer, R. D., and S. K. Raina, 1972: The early ontogeny of embryoids and callus from pollen and subsequent organogenesis in anther culture ofDatura metel and rice. Planta104, 146–156.Google Scholar Kohlenbach, H. W., und T. Geier, 1972: Embryonen ausin vitro kultivierten Antheren von Datura meteloides Dun.,Datura wrightii Regel und Solanum tuberosum L. Z. Pflanzenphysiol.67, 161–165. Narayanaswamy, S., and L.P. Chandy, 1971:In vitro production of haploid, diploid and triploid androgenic embryoids and plantlets in Datura metel L. Ann. Bot.35, 535–542. Nitsch, J. P., and C. Nitsch, 1970: Obtention de plantes haploides à partir de pollen. Bull. Soc. bot. Fr. 117, 339–360. Rajasekhar, E.W., 1973: Nuclear divisions in protoplasts isolated from pollen tetrads of Datura metel. Nature (Lond.)246, 223–224. Satina, S., 1950: Preferential functioning of 2 n pollen grains in certain species of Datura. Amer. J. Bot.37, 666–667. Satina, S.,and A.F. Blakeslee, 1935: Cytological effects of a gene inDatura which causes dyad formation in sporogenesis. Bot. Gaz. 96, 521–523. Sunderland, N., 1973: Pollen and anther culture. In: Plant tissue and cell culture, pp. 205–239. (H. E. Street, ed.). Oxford: Blackwell. Sunderland, N., and F.M. Wicks, 1971: Embryoid formation in pollen grains of Nicotiana tabacum. J. exp. Bot.22, 213–226. Sunderland, N., G. B. Collins, and J.M. Dunwell, 1974: The role of nuclear fusion in pollen embryogenesis of Datura innoxia Mill. Planta 117, 227–241.

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