Griseofulvin solvate solid dispersions with synergistic effect against fungal biofilms

Al-Obaidi, H. ORCID: https://orcid.org/0000-0001-9735-0303, Kowalczyk, R. M., Kalgudi, R. and Zariwala, M. G. (2019) Griseofulvin solvate solid dispersions with synergistic effect against fungal biofilms. Colloids and Surfaces B: Biointerfaces, 184. 11540. ISSN 09277765 doi: 10.1016/j.colsurfb.2019.110540

Abstract/Summary

Fungal biofilms are invariably recalcitrant to antifungal drugs and thus can cause recurrent serious infections. The aim of this work was to prepare highly effective form of the antifungal drug griseofulvin using the chloroform solvate embedded into different polymeric matrices. Based on their solid solubility, solvated (chloroform) and non-solvated (methanol and acetone) solid dispersions were prepared using different materials: silica, microcrystalline cellulose, polyvinylpyrrolidone and hydroxypropyl methylcellulose acetate succinate (HPMCAS) by which HPMCAS dispersions showed the highest solubility of about 200 μg/mL compared with ∼30 μg/mL for pure griseofulvin. The anti fungal potential of griseofulvin was assessed against the dermatophytes T. rubrum. Metabolic and protease activity of T. rubrum NCPF 935 with and without the presence of GF:HPMCAS chloroform solvates showed significant reduction compared to the untreated control after 24 h period. Confocal laser scanning microscopy showed thin hyphae compared to Control and GF:HPMCAS (non solvated). Dynamic vapour sorption data showed that HPMCAS formed most stable solvate structure preventing recrystallization and solvate expulsion, which could explain the disruptive effect of the biofilms. This could be explained by the formed hydrogen bonds as revealed by the solid and liquid state NMR data, which was further confirmed via thermal and FTIR analyses.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/86565
Identification Number/DOI	10.1016/j.colsurfb.2019.110540
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy
Publisher	Elsevier
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