Global transcript analysis of rice leaf and seed using SAGE technology.

Gibbings, J. G., Cook, B. P., Dufault, M. R., Madden, S. L., Khuri, S., Turnbull, C. J. ORCID: https://orcid.org/0009-0007-6470-6933 and Dunwell, J. M. ORCID: https://orcid.org/0000-0003-2147-665X (2003) Global transcript analysis of rice leaf and seed using SAGE technology. Plant Biotechnology Journal, 1 (4). pp. 271-85. ISSN 1467-7652 doi: 10.1046/j.1467-7652.2003.00026.x

Abstract/Summary

We have compiled two comprehensive gene expression profiles from mature leaf and immature seed tissue of rice (Oryza sativa ssp. japonica cultivar Nipponbare) using Serial Analysis of Gene Expression (SAGE) technology. Analysis revealed a total of 50 519 SAGE tags, corresponding to 15 131 unique transcripts. Of these, the large majority (approximately 70%) occur only once in both libraries. Unexpectedly, the most abundant transcript (approximately 3% of the total) in the leaf library was derived from a type 3 metallothionein gene. The overall frequency profiles of the abundant tag species from both tissues differ greatly and reveal seed tissue as exhibiting a non-typical pattern of gene expression characterized by an over abundance of a small number of transcripts coding for storage proteins. A high proportion ( approximately 80%) of the abundant tags (> or = 9) matched entries in our reference rice EST database, with many fewer matches for low abundant tags. Singleton transcripts that are common to both tissues were collated to generate a summary of low abundant transcripts that are expressed constitutively in rice tissues. Finally and most surprisingly, a significant number of tags were found to code for antisense transcripts, a finding that suggests a novel mechanism of gene regulation, and may have implications for the use of antisense constructs in transgenic technology.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/7943
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Ecology and Evolutionary Biology
Uncontrolled Keywords	SAGE;rice;transcript;antisense;seed;leaf
Publisher	Wiley-Blackwell
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