Precise quantification of platelet proteins and their phosphorylation states

Mazet, F. and Fry, M. J. (2018) Precise quantification of platelet proteins and their phosphorylation states. In: Gibbins, J. M. ORCID: https://orcid.org/0000-0002-0372-5352 and Mahaut-Smith, M. (eds.) Platelets and Megakaryocytes: Volume 4, Advanced Protocols and Perspectives. Humana Press, New York, pp. 113-125. ISBN 9781493985845 doi: 10.1007/978-1-4939-8585-2_7

Abstract/Summary

Systems biology and modeling approaches require quantitative data-rich temporal experiments to better understand the dynamics and regulation of the components of the signaling pathways that governs cell biology and physiology. Here we present a modified Western blotting method to rapidly analyze and accurately quantify protein copy number, and their respective phosphorylation states at specific sites over detailed time courses.

Item Type	Book or Report Section
URI	https://reading-clone.eprints-hosting.org/id/eprint/79193
Item Type	Book or Report Section
Refereed	No
Divisions	Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
Uncontrolled Keywords	High-density, Immunoblotting, Protein quantification, Western
Publisher	Humana Press
Download/View statistics	View download statistics for this item