Talin is a substrate for SUMOylation in migrating cancer cells

Huang, Z., Barker, D., Gibbins, J. M. ORCID: https://orcid.org/0000-0002-0372-5352 and Dash, P. R. (2018) Talin is a substrate for SUMOylation in migrating cancer cells. Experimental Cell Research, 370 (2). pp. 417-425. ISSN 0014-4827 doi: 10.1016/j.yexcr.2018.07.005

Abstract/Summary

Focal adhesions (FAs) play an important role in cancer cell migration and metastasis by linking the actin cytoskeleton to the extracellular matrix, allowing the cell to generate traction. SUMOylation is a post-translational modification of proteins on lysine residues that can affect protein localisation, turnover and protein-protein interactions. In this study, we demonstrate that talin, a key component of FAs, can be post-translationally modified by SUMOylation in MDA-MB-231 breast cancer cells and U2OS osteosarcoma cells. Furthermore we demonstrate that SUMOylation regulates the dynamic activities of FAs including their number, size and turnover rate. Inhibiting SUMOylation significantly reduced the speed of cell migration. The identification of talin as a SUMO target provides insight into the mechanisms regulating focal adhesion formation and turnover and potentially identifies a novel mechanism underlying cell migration.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/78268
Identification Number/DOI	10.1016/j.yexcr.2018.07.005
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	Elsevier
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