Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels

Azoidis, I., Metcalfe, J., Reynolds, J., Keeton, S., Hakki, S. S., Sheard, J. and Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X (2017) Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels. MRS Communications, 7 (3). pp. 458-465. ISSN 2159-6859 doi: 10.1557/mrc.2017.59

Abstract/Summary

Human mesenchymal stem cells (MSCs) are the most intensely studied and clinically used adult stem cell type. Conventional long-term cultivation of MSCs as a monolayer is known to result in a reduction of their functionality and viability. In addition, large volumes of cell culture medium are required to obtain cell quantities needed for their clinical use. In this proof of concept study, we cultivated human MSCs within a 3D nanofibrillar cellulose (NFC) hydrogel. We show that NFC is biocompatible with human MSCs, and represents a feasible approach to upscaling of their culture.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/71458
Identification Number/DOI	10.1557/mrc.2017.59
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	Cambridge University Press
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