Comparison of cytokine production in cultures of whole human blood and purified mononuclear cells

Yaqoob, P. ORCID: https://orcid.org/0000-0002-6716-7599, Newsholme, E. and Calder, P. (1998) Comparison of cytokine production in cultures of whole human blood and purified mononuclear cells. Cytokine, 11 (8). pp. 600-605. ISSN 1096-0023

Abstract/Summary

In order to examine inter- and intra-individual variations in cytokine production, blood was collected from 48 healthy subjects on each of 4 occasions separated by 4 weeks. Whole blood (diluted 1:10) and mononuclear cell (MNC) cultures were stimulated for 24 h with either concanavalin A (Con A) or bacterial lipopolysaccharide (LPS) and the concentrations of IL-1.