APOLIPOPROTEIN E (epsilon) genotype has a greater impact on apoB-48 than apoB-100 responses to dietary fat manipulation- insights from the SATgenε study

Jackson, K. G. ORCID: https://orcid.org/0000-0002-0070-3203, Lockyer, S., Carvalho-Wells, A. L., Williams, C. M., Minihane, A. M. and Lovegrove, J. A. ORCID: https://orcid.org/0000-0001-7633-9455 (2017) APOLIPOPROTEIN E (epsilon) genotype has a greater impact on apoB-48 than apoB-100 responses to dietary fat manipulation- insights from the SATgenε study. Molecular Nutrition and Food Research, 61 (4). 1600688. ISSN 1613-4133 doi: 10.1002/mnfr.201600688

Abstract/Summary

SCOPE: To determine the contribution of intestinally and liver-derived lipoproteins to the postprandial plasma triacylglycerol (TAG) response in APOE3/E3 and E3/E4 individuals following chronic dietary fat manipulation. METHODS AND RESULTS: In sequential order, participants (n = 12 E3/E3, n = 11 E3/E4) followed low fat (LF); high-fat, high-saturated fat (HSF); and HSF with 3.45 g/day docosahexaenoic acid (HSF-DHA) diets, each for 8 weeks. After each dietary period, an acute test meal with a macronutrient profile representative of the dietary intervention was consumed. Apolipoprotein (apo)B isoforms were determined in isolated TAG-rich lipoprotein fractions (Sf >400, Sf 60-400 and Sf 20-60) by specific ELISA. A genotype*meal/diet interaction for the Sf >400 fraction apoB-48 response (P<0.05) was observed, with higher concentrations reached after the LF than HSF-DHA meal in E4 carriers. This finding was associated with a lower TAG content of the Sf >400 particles. Fasting Sf 60-400 and 20-60 apoB-48 concentrations were also significantly higher in E4 carriers. No impact of genotype on the apoB-100 responses was evident. CONCLUSION: Our study revealed marked effects of dietary fat composition on the Sf >400 apoB-48 response and particle TAG content in E4 carriers relative to the 'wild-type' E3/E3 genotype, which suggest APOE genotype is a potential modulator of chylomicron particle synthesis. This article is protected by copyright. All rights reserved.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/68386
Identification Number/DOI	10.1002/mnfr.201600688
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR) Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
Publisher	Wiley
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