Mediation of coffee-induced improvements in human vascular function by chlorogenic acids and its metabolites: two randomized, controlled, crossover intervention trials

Mills, C. E. ORCID: https://orcid.org/0000-0002-8313-3700, Flury, A., Marmet, C., Poquet, L., Rimaldi, S. F., Sartori, C., Rexhag, E., Brenner, R., Allman, Y., Zimmerman, D., Gibson, G. R. ORCID: https://orcid.org/0000-0002-0566-0476, Mottram, D. S., Oruna-Concha, M.-J. ORCID: https://orcid.org/0000-0001-7916-1592, Actis-Goretta, L. and Spencer, J. P. E. ORCID: https://orcid.org/0000-0003-2931-7274 (2017) Mediation of coffee-induced improvements in human vascular function by chlorogenic acids and its metabolites: two randomized, controlled, crossover intervention trials. Clinical Nutrition, 36 (6). pp. 1520-1529. ISSN 0261-5614 doi: 10.1016/j.clnu.2016.11.013

Abstract/Summary

Background & aims Polyphenol intake has been linked to improvements in human vascular function, although data on hydroxycinnamates, such as chlorogenic acid (CGA) have not yet been studied. We aimed to investigate the impact of coffee intake rich in chlorogenic acid on human vascular function and whether CGAs are involved in potential effects. Methods Two acute randomized, controlled, cross-over human intervention trials were conducted. The impact of coffee intake, matched for caffeine but differing in CGA content (89, and 310 mg) on flow-mediated dilation (FMD) was assessed in 15 healthy male subjects. In a second intervention trial conducted with 24 healthy male subjects, the impact of pure 5-caffeoylquinic acid (5-CQA), the main CGA in coffee (5-CQA; 450 mg and 900 mg) on FMD was also investigated. Results We observed a bi-phasic FMD response after low and high polyphenol, (89 mg and 310 mg CGA) intake, with increases at 1 (1.10 ± 0.43% and 1.34 ± 0.62%, respectively) and 5 (0.79% ± 0.32 and 1.52% ± 0.40, respectively) hours post coffee consumption. FMD responses to coffee intake was closely paralleled by the appearance of CGA metabolites in plasma, notably 3-, 4- and 5-CQA and ferulic-4′-O-sulfate at 1 h and isoferulic-4′-O-glucuronide and ferulic-4′-O-sulfate at 5 h. Intervention with purified 5-CQA (450 mg) also led to an improvement in FMD response relative to control (0.75 ± 1.31% at 1 h post intervention, p = 0.06) and concomitant appearance of plasma metabolites. Conclusions Coffee intake acutely improves human vascular function, an effect, in part, mediated by 5-CQA and its physiological metabolites.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/68297
Identification Number/DOI	10.1016/j.clnu.2016.11.013
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
Uncontrolled Keywords	Coffee; Chlorogenic acid; Vascular; Phenolics; Flow mediated dilatation (FMD)
Publisher	Elsevier
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