Liquid MALDI MS analysis of complex peptide and proteome samples

Wiangnon, K. and Cramer, R. ORCID: https://orcid.org/0000-0002-8037-2511 (2016) Liquid MALDI MS analysis of complex peptide and proteome samples. Journal of Proteome Research, 15 (9). pp. 2998-3008. ISSN 1535-3907 doi: 10.1021/acs.jproteome.6b00148

Abstract/Summary

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) is well-known to be a powerful technique for the analysis of biological samples. By using glycerol-based liquid support matrices (LSMs) instead of conventional MALDI matrices the power of this technique can be extended further. In this study, we exploited LSMs for the identification of complex samples, i.e. the Lactobacillus proteome and a bovine serum albumin (BSA) digest. Liquid and solid MALDI samples were manually and robotically prepared by coupling a nanoflow high performance liquid chromatography (nanoHPLC) system to an automated MALDI sample spotting device. MS and MS/MS data were successfully acquired at the femtomole level using TOF/TOF as well as Q-TOF instrumentation and used for protein identification searching sequence databases. For the BSA digest analysis, liquid MALDI samples resulted in peptide mass fingerprints which led to a higher confidence in protein identification compared to solid (crystalline) MALDI samples. However, post-source decay (PSD) MS/MS analysis of both the proteome of Lactobacillus plantarum WCFS1 cells and BSA digest showed that further optimization of the formation and detection of peptide fragment ions is still needed for liquid MALDI samples as the MS/MS ion search score was lower than for the solid MALDI samples, reflecting the poorer quality of the liquid MALDI-PSD spectra, which can be attributed to the differences in PSD parameters and their optimization that is currently achievable.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/66159
Identification Number/DOI	10.1021/acs.jproteome.6b00148
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Mass Spectrometry (CAF)
Uncontrolled Keywords	liquid support matrix, liquid MALDI, MALDI-TOF, QTOF, mass spectrometry, LCMALDI, automation, MALDI-PSD, MALDI-CID, MALDI MS/MS
Publisher	American Chemical Society
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