Lab on a stick: multi-analyte cellular assays in a microfluidic dipstick

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Reis, N. M., Pivetal, J., Loo-Zazueta, A. L., Barros, J. M. S. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2016) Lab on a stick: multi-analyte cellular assays in a microfluidic dipstick. Lab on a Chip, 16 (15). pp. 2891-2899. ISSN 1473-0189 doi: 10.1039/C6LC00332J

Abstract/Summary

A new microfluidic concept for multi-analyte testing in a dipstick format is presented, termed “Lab-on-a-Stick”, that combines the simplicity of dipstick tests with the high performance of microfluidic devices. Lab-on-a-Stick tests are ideally suited to analysis of particulate samples such as mammalian or bacterial cells, and capable of performing multiple different parallel microfluidic assays when dipped into a single sample with results recorded optically. The utility of this new diagnostics format was demonstrated by performing three types of multiplex cellular assays that are challenging to perform in conventional dipsticks: 1) instantaneous ABO blood typing; 2) microbial identification; and 3) antibiotic minimum inhibitory (MIC) concentration measurement. A pressure balance model closely predicted the superficial flow velocities in individual capillaries, that were overestimated by up to one order of magnitude by the Lucas-Washburn equation conventionally used for wicking in cylindrical pores. Lab-on-a-stick provides a cost-effective, simple, portable and flexible multiplex platform for a range of assays, and will deliver a new generation of advanced yet affordable point-of-care tests for global diagnostics.

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Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/66028
Identification Number/DOI	10.1039/C6LC00332J
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Mass Spectrometry (CAF) Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
Publisher	Royal Society of Chemistry
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Funders:	Engineering and Physical Sciences Research Council	The sponsoring bodies who contributed funding for the creation of this item. Example: NERC Example: The Royal Society of Chemistry A pick list of funders may appear as you type in the funder's name in full or as an acronym. Select a correct match to complete the field or type in a new entry in full. For new entries, the full name is preferred.
Projects:	Lab on a stick: Smartphone-ready low cost rapid live microbe detection and identification using nanocoated Micro Capillary Film Funded by: Engineering and Physical Sciences Research Council (EP/L013983/1 - £98,491) Local Lead (PI): Al Edwards Project Lead: Alexander Daniel Edwards 1 April 2014 - 30 June 2015	Click Add to select your project (received at Reading) from an autocomplete list.

Deposit Details

Date Deposited:	15 Jul 2016 11:48	Date item deposited into CentAUR
Last Modified:	23 Jun 2024 05:32	Date item last modified

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