Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots

Ong, W. K., Tan, C. S., Chan, K. L., Goesantoso, G. G., Chan, X. H. D., Chan, E., Yin, J., Chia, R. Y., Khoo, C. M., So, J. B. Y., Shabbir, A., Toh, S.-A., Han, W. and Sugii, S. (2014) Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots. Stem Cell Reports, 2 (2). pp. 171-179. ISSN 2213-6711 doi: 10.1016/j.stemcr.2014.01.002

Abstract/Summary

Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/65855
Identification Number/DOI	10.1016/j.stemcr.2014.01.002
Refereed	Yes
Divisions	University of Reading Malaysia
Publisher	Elsevier
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