Using NMR-based metabolomics to evaluate postprandial urinary responses following consumption of minimally processed wheat bran or wheat aleurone by men and women

Garg, R., Brennan, L., Price, R. K., Wallace, J. M. W., Strain, J. J., Gibney, M. J., Shewry, P. R., Ward, J. L., Garg, L. and Welch, R. W. (2016) Using NMR-based metabolomics to evaluate postprandial urinary responses following consumption of minimally processed wheat bran or wheat aleurone by men and women. Nutrients, 8 (2). 96. ISSN 2072-6643 doi: 10.3390/nu8020096

Abstract/Summary

Wheat bran, and especially wheat aleurone fraction, are concentrated sources of a wide range of components which may contribute to the health benefits associated with higher consumption of whole-grain foods. This study used NMR metabolomics to evaluate urine samples from baseline at one and two hours postprandially, following the consumption of minimally processed bran, aleurone or control by 14 participants (7 Females; 7 Males) in a randomized crossover trial. The methodology discriminated between the urinary responses of control, and bran and aleurone, but not between the two fractions. Compared to control, consumption of aleurone or bran led to significantly and substantially higher urinary concentrations of lactate, alanine, N-acetylaspartate acid and N-acetylaspartylglutamate and significantly and substantially lower urinary betaine concentrations at one and two hours postprandially. There were sex related differences in urinary metabolite profiles with generally higher hippurate and citrate and lower betaine in females compared to males. Overall, this postprandial study suggests that acute consumption of bran or aleurone is associated with a number of physiological effects that may impact on energy metabolism and which are consistent with longer term human and animal metabolomic studies that used whole-grain wheat diets or wheat fractions.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/62838
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Uncontrolled Keywords	NMR metabolomics; wheat bran; wheat aleurone; energy metabolism; sex differences; urine; lactate; alanine; N-acetylaspartate; N-acetylaspartylglutamate; betaine; hippurate
Publisher	MDPI
Download/View statistics	View download statistics for this item