Regulation of mitogen-activated protein kinase cascades in the heart.

Sugden, P. H. and Clerk, A. ORCID: https://orcid.org/0000-0002-5658-0708 (1998) Regulation of mitogen-activated protein kinase cascades in the heart. Advances in enzyme regulation, 38. pp. 87-98. ISSN 0065-2571

Abstract/Summary

Using primary cultures of neonatal rat ventricular myocytes and isolated adult rat hearts as models, we have characterized extensively the regulation of MAPKs in the heart. The ERKs are activated primarily by GPCR agonists acting through PKC. These agonists can also activate the JNKs although the mechanism is unclear. Cellular stresses stimulate strong activation of the JNKs, but also cause some stimulation of ERKs. Activation of p38-MAPK has so far only been demonstrated in intact adult hearts subjected to stresses and probably leads to activation of MAPKAPK2. Both cellular stresses and GPCR agonists induce phosphorylation of c-Jun, but only the latter causes upregulation of c-Jun protein.