Poulter, N. S., Pollitt, A. Y. ORCID: https://orcid.org/0000-0001-8706-5154, Davies, A., Malinova, D., Nash, G. B., Hannon, M. J., Pikramenou, Z., Rappoport, J. Z., Hartwig, J. H., Owen, D. M., Thrasher, A. J., Watson, S. P. and Thomas, S. G.
(2015)
Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich syndrome protein and ARP2/3 complex.
Nature Communications, 6.
7254.
ISSN 2041-1723
doi: 10.1038/ncomms8254
Abstract/Summary
The actin nodule is a novel F-actin structure present in platelets during early spreading. However, only limited detail is known regarding nodule organization and function. Here we use electron microscopy, SIM and dSTORM super-resolution, and live-cell TIRF microscopy to characterize the structural organization and signalling pathways associated with nodule formation. Nodules are composed of up to four actin-rich structures linked together by actin bundles. They are enriched in the adhesion-related proteins talin and vinculin, have a central core of tyrosine phosphorylated proteins and are depleted of integrins at the plasma membrane. Nodule formation is dependent on Wiskott-Aldrich syndrome protein (WASp) and the ARP2/3 complex. WASp(-/-) mouse blood displays impaired platelet aggregate formation at arteriolar shear rates. We propose actin nodules are platelet podosome-related structures required for platelet-platelet interaction and their absence contributes to the bleeding diathesis of Wiskott-Aldrich syndrome.
Altmetric Badge
Item Type | Article |
URI | https://reading-clone.eprints-hosting.org/id/eprint/44786 |
Item Type | Article |
Refereed | Yes |
Divisions | Life Sciences > School of Biological Sciences > Biomedical Sciences |
Publisher | Nature Publishing Group |
Download/View statistics | View download statistics for this item |
Downloads
Downloads per month over past year
University Staff: Request a correction | Centaur Editors: Update this record