The proteome of the infectious bronchitis virus Beau-R Virion

Dent, S., Xia, D., Wastling, J., Neuman, B., Britton, P. and Maier, H. (2015) The proteome of the infectious bronchitis virus Beau-R Virion. Journal of General Virology, 96 (12). pp. 3499-3506. ISSN 0022-1317 doi: 10.1099/jgv.0.000304

Abstract/Summary

Infectious bronchitis is a highly contagious respiratory disease of poultry caused by the coronavirus IBV. It was thought that coronavirus virions were composed of three major viral structural proteins, until investigations of other coronaviruses showed that coronavirus virions also include viral non-structural and group specific proteins as well as host cell proteins. To study the proteome of IBV virions, virus was grown in embryonated chicken eggs and purified by sucrose gradient ultracentrifugation and analysed by mass spectrometry proteomic. Analysis of three preparations of purified IBV yielded the three expected structural proteins plus thirty-five additional virion-associated host proteins. Virion-associated host proteins had a diverse range of functional attributions, being involved in cytoskeleton formation, RNA binding and protein folding pathways. Some of these proteins were unique to this study, whilst others were found to be orthologous to proteins identified in SARS-CoV virions, and also virions from a number of other RNA and DNA viruses. Together these results demonstrate that coronaviruses have the capacity to incorporate a substantial variety of host protein, which may have implications for the disease process.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/43569
Identification Number/DOI	10.1099/jgv.0.000304
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	Society of General Microbiology
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