A self-assembling fluorescent dipeptide conjugate for cell labelling

Kirkham, S., Hamley, I. W. ORCID: https://orcid.org/0000-0002-4549-0926, Smith, A. M., Gouveia, R. M., Connon, C. J., Reza, M. and Ruokolainen, J. (2016) A self-assembling fluorescent dipeptide conjugate for cell labelling. Colloids and Surfaces B: Biointerfaces, 137. pp. 104-108. ISSN 0927-7765 doi: 10.1016/j.colsurfb.2015.04.062

Abstract/Summary

Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in peri-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/41591
Identification Number/DOI	10.1016/j.colsurfb.2015.04.062
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	Elsevier
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