Use of TD-GC-TOF-MS to assess volatile composition during post harvest storage in seven accessions of rocket salad (Eruca sativa)

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Bell, L. orcid id iconORCID: https://orcid.org/0000-0003-2895-2030, Spadafora, N. D., Mueller, C. T., Wagstaff, C. orcid id iconORCID: https://orcid.org/0000-0001-9400-8641 and Rogers, H. j. (2016) Use of TD-GC-TOF-MS to assess volatile composition during post harvest storage in seven accessions of rocket salad (Eruca sativa). Food Chemistry, 194. pp. 626-636. ISSN 0308-8146 doi: 10.1016/j.foodchem.2015.08.043

Abstract/Summary

An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD- GC-TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7 days. Relative amounts of VOCs across 3 time points were significantly different - isothiocyanate-containing molecules being abundant on 'Day 0'. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf-life.

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Item Type Article
URI https://reading-clone.eprints-hosting.org/id/eprint/41354
Identification Number/DOI 10.1016/j.foodchem.2015.08.043
Refereed Yes
Divisions Interdisciplinary centres and themes > Centre for Food Security
Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group
Uncontrolled Keywords Eruca sativa; Diplotaxis tenuifolia; Brassicaceae; rocket salad; rucola; gas 28 chromatography mass spectrometry; volatile organic chemicals; isothiocyanates.
Publisher Elsevier
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