Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device

Barbosa, A. I., Gehlot, P., Sidapra, K., Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X and Reis, N. M. (2015) Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device. Biosensors and Bioelectronics, 70. pp. 5-14. ISSN 0956-5663 doi: 10.1016/j.bios.2015.03.006

Abstract/Summary

We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60 ng/ml with < 7 % precision in 13 minutes using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08 ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22 minutes total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/39696
Identification Number/DOI	10.1016/j.bios.2015.03.006
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
Publisher	Elsevier
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