Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons

Klenke, C., Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X, Engelen, T., Müller, J., Noll, T., Niehaus, K., Schmitz, M. L., Kaltschmidt, B. and Kaltschmidt, C. (2013) Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE, 8 (6). e65280. ISSN 1932-6203 doi: 10.1371/journal.pone.0065280

Abstract/Summary

Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/39518
Identification Number/DOI	10.1371/journal.pone.0065280
Refereed	Yes
Divisions	No Reading authors. Back catalogue items Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	Public Library of Science
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