Impact of polysialylated CD56 on natural killer cell cytotoxicity

Moebius, J. M., Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X, Schmitz, J., Kaltschmidt, C. and Piechaczek, C. (2007) Impact of polysialylated CD56 on natural killer cell cytotoxicity. BMC immunology, 8 (1). 13. ISSN 1471-2172 doi: 10.1186/1471-2172-8-13

Abstract/Summary

Background Siglec-7, a sialic acid binding inhibitory receptor expressed by NK cells is masked in vivo by a so far unknown ligand. It shows a strong binding prevalence for α-2,8-linked disialic acids in vitro. Results Here we describe the expression of PSA-NCAM (α-2,8-linked polysialic acid modified NCAM) on functional adult peripheral blood natural killer cells and examine its possible role in masking Siglec-7. Unmasking of Siglec-7 using Clostridium perfringens neuraminidase massively reduces NK cell cytotoxicity. By contrast a specific removal of PSA using Endo-NF does not lead to a reduction of NK cell cytotoxicity. Conclusion The results presented here therefore indicate that PSA-NCAM is not involved in masking Siglec-7.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/39500
Identification Number/DOI	10.1186/1471-2172-8-13
Refereed	Yes
Divisions	No Reading authors. Back catalogue items Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Publisher	BioMed Central
Download/View statistics	View download statistics for this item