The pgip family in soybean and three other legume species: evidence for a birth-and-death model of evolution

Kalunke, R. M., Cenci, A., Volpi, C., O'Sullivan, D. M. ORCID: https://orcid.org/0000-0003-4889-056X, Sella, L., Favaron, F., Cervone, F., De Lorenzo, G. and D’Ovidio, R. (2014) The pgip family in soybean and three other legume species: evidence for a birth-and-death model of evolution. BMC Plant Biology, 14 (1). 189. ISSN 1471-2229 doi: 10.1186/s12870-014-0189-3

Abstract/Summary

Background Polygalacturonase-inhibiting proteins (PGIPs) are leucine-rich repeat (LRR) plant cell wall glycoproteins involved in plant immunity. They are typically encoded by gene families with a small number of gene copies whose evolutionary origin has been poorly investigated. Here we report the complete characterization of the full complement of the pgip family in soybean (Glycine max [L.] Merr.) and the characterization of the genomic region surrounding the pgip family in four legume species. Results BAC clone and genome sequence analyses showed that the soybean genome contains two pgip loci. Each locus is composed of three clustered genes that are induced following infection with the fungal pathogen Sclerotinia sclerotiorum (Lib.) de Bary, and remnant sequences of pgip genes. The analyzed homeologous soybean genomic regions (about 126 Kb) that include the pgip loci are strongly conserved and this conservation extends also to the genomes of the legume species Phaseolus vulgaris L., Medicago truncatula Gaertn. and Cicer arietinum L., each containing a single pgip locus. Maximum likelihood-based gene trees suggest that the genes within the pgip clusters have independently undergone tandem duplication in each species. Conclusions The paleopolyploid soybean genome contains two pgip loci comprised in large and highly conserved duplicated regions, which are also conserved in bean, M. truncatula and C. arietinum. The genomic features of these legume pgip families suggest that the forces driving the evolution of pgip genes follow the birth-and-death model, similar to that proposed for the evolution of resistance (R) genes of NBS-LRR-type.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/37250
Identification Number/DOI	10.1186/s12870-014-0189-3
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Centre for Food Security Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Publisher	BioMed Central
Download/View statistics	View download statistics for this item