Application of DIGE and mass spectrometry in the study of type 2 Diabetes Mellitus (T2DM) mouse models

Smith, C., Mills, D. and Cramer, R. ORCID: https://orcid.org/0000-0002-8037-2511 (2012) Application of DIGE and mass spectrometry in the study of type 2 Diabetes Mellitus (T2DM) mouse models. In: Cramer, R. ORCID: https://orcid.org/0000-0002-8037-2511 and Westermeier, R. (eds.) Difference Gel Electrophoresis (DIGE). Methods in molecular biology (854). Humana Press / Springer, New York, pp. 299-318. ISBN 9781617795725 doi: 10.1007/978-1-61779-573-2_21

Abstract/Summary

Knowledge of the differences between the amounts and types of protein that are expressed in diseased compared to healthy subjects may give an understanding of the biological pathways that cause disease. This is the reasoning behind the presented protocol, which uses difference gel electrophoresis to discover up‐ or down‐regulated proteins between mice of different genotypes, or of those fed on different diets, that may thus be prone to develop diabetes‐like phenotypes. Subsequent analysis of these proteins by tandem mass spectrometry typically facilitates their identification with a high degree of confidence.

Item Type	Book or Report Section
URI	https://reading-clone.eprints-hosting.org/id/eprint/30234
Identification Number/DOI	10.1007/978-1-61779-573-2_21
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	Humana Press / Springer
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