Sulphate transport by rat ileum. Effect of molybdate and other anions

Cardin, C. J. ORCID: https://orcid.org/0000-0002-2556-9995 and Mason, J. (1975) Sulphate transport by rat ileum. Effect of molybdate and other anions. Biochimica et Biophysica Acta (BBA) - Biomembranes, 394 (1). pp. 46-54. ISSN 0005-2736 doi: 10.1016/0005-2736(75)90203-5

Abstract/Summary

Kinetic constants for SO42− transport by upper and lower rat ileum in vitro have been determined by computer fitting of rate vs concentration data obtained using the everted sac technique. MoO42− inhibition of this transport is competitive, and kinetic constants for the inhibition were similarly determined. Transport is also inhibited by the anions WO42−, S2O32− and SeO42−, in the order . These anions have no effect on the transport of l-valine. Low SO42− transport rates were observed in sacs from animals fed a high-molybdenum diet. The significance of the results with respect to the problem of molybdate toxicity in animals is discussed, and related to the known protective effect of SO42−.