Genetic expression of an amyloid peptide fragment and analysis of formylated products

Cheng, G., Krasel, C., Zhou, H. G., Chappell, D. and Hamley, I. W. ORCID: https://orcid.org/0000-0002-4549-0926 (2011) Genetic expression of an amyloid peptide fragment and analysis of formylated products. Organic Letters, 13 (10). pp. 2572-2575. ISSN 1523-7060 doi: 10.1021/ol1028145

Abstract/Summary

The model amyloid peptide AAKLVFF was expressed as a His-tagged fusion protein with the immunoglobulin-binding domain B1 of streptococcal protein G (GB1), a small (56 residues), stable, single-domain protein. It is shown that expression of this model amyloid peptide is possible and is not hindered by aggregation. Formylation side reactions during the CNBr cleavage are investigated via synthesis of selectively formylated peptides.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/20399
Identification Number/DOI	10.1021/ol1028145
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
Publisher	The American Chemical Society
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