Adult skeletal muscle stem cell migration is mediated by a blebbing/amoeboid mechanism

Otto, A., Collins-Hooper, H., Patel, A., Dash, P. R. and Patel, K. (2011) Adult skeletal muscle stem cell migration is mediated by a blebbing/amoeboid mechanism. Rejuvenation Research, 14 (3). pp. 249-260. ISSN 1549-1684 doi: 10.1089/rej.2010.1151

Abstract/Summary

Adult skeletal muscle possesses a resident stem cell population called satellite cells which are responsible for tissue repair following damage. Satellite cell migration is crucial in promoting rapid tissue regeneration but is a poorly understood process. Furthermore, the mechanisms facilitating satellite cell movement have yet to be elucidated. Here the process of satellite cell migration has been investigated revealing that they undergo two distinct phases of movement; firstly under the basal lamina and then rapidly increasing their velocity when on the myofibre surface. Most significantly we show that satellite cells move using a highly dynamic blebbing based mechanism and not via lamellopodia mediated propulsion. We show that nitric oxide and non-canonical Wnt signalling pathways are necessary for regulating the formation of blebs and the migration of satellite cells. In summary, we propose that the formation of blebs and their necessity for satellite cell migration has significant implications in the future development of therapeutic regimes aimed at promoting skeletal muscle regeneration.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/18088
Identification Number/DOI	10.1089/rej.2010.1151
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
Publisher	Mary Ann Liebert
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