Cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors: detection methods and activity measurements

Brooks, G. (2005) Cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors: detection methods and activity measurements. In: Humphrey, T. and Brooks, G. (eds.) Cell cycle control: mechanisms and protocols. Methods in molecular biology, 296. Humana Press, New Jersey, pp. 291-298. ISBN 9781588291448 doi: 10.1385/1-59259-857-9:291

Abstract/Summary

The cyclin/cyclin-dependent kinase (Cdk) complexes and the Cdk inhibitors (CDKI) are crucial regulators of cell cycle progression in all eukaryotic cells. Using rat cardiac myocytes as a model system, this chapter provides a detailed account of methods that can be employed to measure both cyclin/Cdk activity in cells and the extent of CDKI inhibitory activity present in a particular cell type.