Characterisation of cyclin D1 down-regulation in coronavirus infected cells

Harrison, S.M., Dove, B.K., Rothwell, L., Kaiser, P., Tarpey, I., Brooks, G. and Hiscox, J.A. (2007) Characterisation of cyclin D1 down-regulation in coronavirus infected cells. FEBS Letters, 581 (7). pp. 1275-1286. ISSN 0014-5793 doi: 10.1016/j.febslet.2007.02.039

Abstract/Summary

The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.