Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects

Mills, D. J. S., Tuohy, K. M., Booth, J., Buck, M., Crabbe, M. J. C., Gibson, G. R. ORCID: https://orcid.org/0000-0002-0566-0476 and Ames, J. M. (2008) Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects. Journal of Applied Microbiology, 105 (3). pp. 706-714. ISSN 1364-5072 doi: 10.1111/j.1365-2672.2008.03783.x

Abstract/Summary

Aim: To investigate the effect of native, heated and glycated bovine serum albumin (BSA) on the ulcerative colitis (UC) and non-UC colonic microbiota in vitro. Methods and Results: Continuous flow culture (CFC) models of the human colonic microbiota inoculated with faeces from UC and non-UC volunteers were maintained on BSA as growth substrate. Changes in bacterial populations and short-chain fatty acids were determined. UC and non-UC microbiota differed significantly in microbial populations, with elevated numbers of sulfate-reducing bacteria (SRB) and clostridia in the microbiota from UC patients. Compared with native BSA, glycated BSA modulated the gut microbiota of UC patients in vitro towards a more detrimental community structure with significant increases in putatively harmful bacteria (clostridia, bacteroides and SRB; P < 0.009) and decreases in dominant and putatively beneficial bacterial groups (eubacteria and bifidobacteria; P < 0.0004). The levels of beneficial short-chain fatty acids were significantly decreased by heated or glycated BSA, but were increased significantly by native BSA. Conclusion: The UC colonic microbiota maintained in CFC was significantly modified by glycated BSA. Significance and Impact of the Study: Results suggest that dietary glycated protein may impact upon the composition and activity of the colonic microbiota, an important environmental variable in UC.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/13146
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Uncontrolled Keywords	colonic microbiota, continuous flow culture, glycated protein, short-chain fatty acids, sulfate-reducing bacteria, ulcerative colitis, IN-SITU HYBRIDIZATION, MAILLARD REACTION-PRODUCTS, OLIGONUCLEOTIDE, PROBES, BACTERIAL-POPULATIONS, METABOLISM, DISEASE, SYSTEM, FLORA, VITRO, MICE
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