Effect of pH and dose on the growth of gut bacteria on prebiotic carbohydrates in vitro

Palframan, R., Gibson, G.R. ORCID: https://orcid.org/0000-0002-0566-0476 and Rastall, R.A. (2003) Effect of pH and dose on the growth of gut bacteria on prebiotic carbohydrates in vitro. Anaerobe, 8 (5). pp. 287-292. ISSN 1075-9964 doi: 10.1006/anae.2002.0434

Abstract/Summary

The effect of pH and substrate dose on the fermentation profile of a number of commercial prebiotics was analysed in triplicate using stirred, pH and temperature controlled anaerobic batch culture fermentations, inoculated with a fresh faecal slurry from one of three healthy volunteers. Bacterial numbers were enumerated using fluorescence in situ hybridisation. The commercial prebiotics investigated were fructooligosaccharides (FOS), inulin, galactooligosaccharides (GOS), isomaltooligosaccharides (IMO) and lactulose. Two pH values were investigated, i.e. pH 6 and 6.8. Doses of 1% and 2% (w/v) were investigated, equivalent to approximately 4 and 8 g per day, respectively, in an adult diet. It was found that both pH and dose altered the bacterial composition. It was observed that FOS and inulin demonstrated the greatest bifidogenic effect at pH 6.8 and 1% (w/v) carbohydrate, whereas GOS, IMO and lactulose demonstrated their greatest bifidogenic effect at pH 6 and 2% (w/v) carbohydrate. From this we can conclude that various prebiotics demonstrate differing bifidogenic effects at different conditions in vitro. (C) 2003 Elsevier Science Ltd. All rights reserved.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/13097
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Uncontrolled Keywords	prebiotic, bifidobacteria, fructooligosaccharides (FOS), inulin, galactooligosaccharides (GOS), isomaltooligosaccharides (IMO), lactulose , IN-SITU HYBRIDIZATION, OLIGONUCLEOTIDE PROBES, BETA-GALACTOSIDASE, DIETARY MODULATION, BIFIDOBACTERIA, PROBIOTICS, MICROFLORA, SYSTEM, COLON
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