Comparison of prebiotic candidates in ulcerative colitis using an in vitro fermentation model

Kennedy, J. M. ORCID: https://orcid.org/0000-0001-9335-0808, De Silva, A., Walton, G. E. ORCID: https://orcid.org/0000-0001-5426-5635, Poveda, C. and Gibson, G. R. ORCID: https://orcid.org/0000-0002-0566-0476 (2024) Comparison of prebiotic candidates in ulcerative colitis using an in vitro fermentation model. Journal of Applied Microbiology, 135 (2). lxae034. ISSN 1365-2672 doi: 10.1093/jambio/lxae034

Abstract/Summary

Aims This study explored the effect of three different prebiotics, the human milk oligosaccharide (HMO) 2’-Fucosyllactose (2’-FL), an oligofructose-enriched inulin (fructo-oligosaccharide, or FOS), and a galacto-oligosaccaride (GOS) mixture, on the faecal microbiota from patients with ulcerative colitis (UC) using in vitro batch culture fermentation models. Changes in bacterial groups and short-chain fatty acid (SCFA) production were compared. Methods and Results In vitro, pH controlled batch culture fermentation was carried out over 48 hours on samples from three healthy controls and three patients with active UC. Four vessels were ran, one negative control and one for each of the prebiotic substrates. Bacterial enumeration was carried out using fluorescence in situ hybridisation with flow cytometry. SCFA quantification was performed using gas chromatography mass spectrometry. All substrates had a positive effect on the gut microbiota and led to significant increases in total SCFA and propionate concentrations at 48 hours. 2’-FL was the only substrate to significantly increase acetate and led to the greatest increase in total SCFA concentration at 48 hours. 2’-FL best suppressed Desulfovibrio spp., a pathogen associated with UC. Conclusions 2’FL, FOS and GOS all significantly improved the gut microbiota in this in vitro study and also led to increased SCFA.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/115280
Identification Number/DOI	10.1093/jambio/lxae034
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
Uncontrolled Keywords	Applied Microbiology and Biotechnology, General Medicine, Biotechnology
Publisher	Oxford University Press (OUP)
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