Genome-wide association study reveals candidate genes controlling root system architecture under low phosphorus supply at seedling stage in Brassica napus

Yuan, P., Liu, H., Wang, X., Hammond, J. P. ORCID: https://orcid.org/0000-0002-6241-3551 and Shi, L. ORCID: https://orcid.org/0000-0002-5312-8521 (2023) Genome-wide association study reveals candidate genes controlling root system architecture under low phosphorus supply at seedling stage in Brassica napus. Molecular Breeding, 43 (8). 63. ISSN 1380-3743 doi: 10.1007/s11032-023-01411-2

Abstract/Summary

Optimal root system architecture (RSA) is essential for vigorous growth and yield in crops. Plants have evolved adaptive mechanisms in response to low phosphorus (LP) stress, and one of those is changes in RSA. Here, more than five million single-nucleotide polymorphisms (SNPs) obtained from whole-genome re-sequencing data (WGR) of an association panel of 370 oilseed rape ( L.) were used to conduct a genome-wide association study (GWAS) of RSA traits of the panel at LP in "pouch and wick" system. Fifty-two SNPs were forcefully associated with lateral root length (LRL), total root length (TRL), lateral root density (LRD), lateral root number (LRN), mean lateral root length (MLRL), and root dry weight (RDW) at LP. There were significant correlations between phenotypic variation and the number of favorable alleles of the associated loci on chromosomes A06 (chrA06_20030601), C03 (chrC03_3535483), and C07 (chrC07_42348561), respectively. Three candidate genes ( , , and ) were detected by combining transcriptome, candidate gene association analysis, and haplotype analysis. Cultivar carrying "CCGC" at , "CAAT" at , and "ATC" at had greater LRL, LRN, and RDW than lines carrying other haplotypes at LP supply. The RSA of a cultivar harboring the three favorable haplotypes was further confirmed by solution culture experiments. These findings define exquisite insights into genetic architectures underlying RSA at LP and provide valuable gene resources for root breeding.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/112938
Identification Number/DOI	10.1007/s11032-023-01411-2
Refereed	Yes
Divisions	Life Sciences > School of Agriculture, Policy and Development > Farm Management Unit
Publisher	Springer Verlag
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