Gamma aminobutyric acid production by commercially available probiotic strains

Monteagudo-Mera, A., Fanti, V., Rodriguez-Sobstel, C., Gibson, G. ORCID: https://orcid.org/0000-0002-0566-0476, Wijeyesekera, A. ORCID: https://orcid.org/0000-0001-6151-5065, Karatzas, K.-A. and Chakrabarti, B. ORCID: https://orcid.org/0000-0002-6649-7895 (2023) Gamma aminobutyric acid production by commercially available probiotic strains. Journal of Applied Microbiology, 134 (2). lxac066. ISSN 1364-5072 doi: 10.1093/jambio/lxac066

Abstract/Summary

Aims Certain bacteria can produce gamma aminobutyric acid (GABA) from glutamate in the human intestinal tract, leading to the possibility of altering GABA levels through diet. To this end, we assessed the ability of seven commercially available probiotic supplements to produce GABA. Method and results Probiotic strains were compared for GABA production in pure culture. The bacteria were inoculated at a concentration of 107 CFU ml−1 in 10 ml MRS supplemented with monosodium glutamate (1% w/v), both with and without oligofructose-enriched inulin (OFI) (1% w/v). Two strains with the highest production of GABA were further assessed for 48 h in pH-controlled anaerobic batch cultures inoculated with faecal bacteria. Liquid chromatography-mass spectrometry (LC–MS) was used for quantification of GABA and microbiota composition was determined through 16S rRNA gene sequencing. Levilactobacillus brevis LB01 (CGMCC 16921) and Lactiplantibacillus plantarum 299v (DSM 9843) were the most efficient producers of GABA. High GABA levels (28.32 mmol l−1 ± 0.29) were produced by the probiotic strain L. brevis LB01 at pH 5.4–5.6. This was significantly higher than the levels of GABA produced by L. plantarum (4.8 mmol l−1 ± 6.8) and a negative control (2.9 mM ± 3.1). The addition of OFI did not further stimulate GABA production under the conditions tested. The ability of these strains to produce GABA in-vitro was further evaluated in a faecal microbiota environment. Once again, L.brevis LB01 produced the highest levels of GABA (40.24 mmol l−1 ± 20.98). Conclusions L. brevis LB01 was found to be the most efficient probiotic strain, of those tested, for GABA production.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/109605
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Psychology and Clinical Language Sciences > Department of Psychology Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
Publisher	The Society for Applied Microbiology
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