Biomolecular corona stability in association with plasma cholesterol level

Trinh, D. N., Radlinskaite, M., Cheeseman, J., Kuhnle, G. ORCID: https://orcid.org/0000-0002-8081-8931, Osborn, H. M. I. ORCID: https://orcid.org/0000-0002-0683-0457, Meleady, P., Spencer, D. I. R. and Monopoli, M. P. (2022) Biomolecular corona stability in association with plasma cholesterol level. Nanomaterials, 12 (15). 2661. ISSN 2079-4991 doi: 10.3390/nano12152661

Abstract/Summary

Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studies use either blood plasma or serum from a pooled biobank, it is unclear whether differences in the media, such as cholesterol level or protein concentration, might affect the NP colloidal stability and corona composition. In this study, the silica corona was prepared at particularly low plasma concentrations (3%, v/v–1.98 mg/mL) to identify the critical roles of the protein mass/NP surface ratio and the level of plasma cholesterol on the corona protein pattern and particle stability. While depending on the plasma dilution factor, the corona protein composition could be controlled by keeping the protein/NP constant. The NP colloidal stability was found to strongly correlate with the level of cholesterol in human plasma, particularly due to the high enrichment of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the corona. A cohort study on plasma samples from individuals with known cholesterol levels was performed to highlight that association, which could be relevant for all corona systems enriched with the LDL.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/106462
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Human Nutrition Research Group Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Medicinal Chemistry Research Group
Publisher	MDPI
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