Molecular comparisons of the Culex pipiens (L.) complex esterase gene amplicons

Buss, D. S. and Callaghan, A. ORCID: https://orcid.org/0000-0002-2731-3352 (2004) Molecular comparisons of the Culex pipiens (L.) complex esterase gene amplicons. Insect Biochemistry and Molecular Biology, 34 (5). pp. 433-441. ISSN 0965-1748 doi: 10.1016/j.ibmb.2004.02.002

Abstract/Summary

The amplification of carboxylesterase genes is a mechanism of organophosphate resistance in Culex mosquitoes. Amplified carboxylesterase genes from an insecticide resistant Culex pipiens strain collected in Cyprus were analysed and compared to other Culex amplified carboxylesterase alleles. A 12 kb section of genomic DNA containing two gene loci coding for carboxylesterase alleles A5 and B5 was cloned and sequenced. A comparison between this amplicon and one from a strain with co-amplified carboxylesterase alleles A2 and B2 revealed a number of differences. The intergenic spacer was 3.7 kb in length in the A5-B5 amplicon (2.7 kb in A2-B2) and contained putative Juan and transposable elements upstream of B5. A fragment of a gene with high homology to aldehyde oxidase was also present immediately downstream of A5. The comparison revealed no differences that would explain the successful spread of the A2-B2 amplicon worldwide whilst the A5-B5 amplicon is restricted to the Mediterranean. (C) 2004 Elsevier Ltd. All rights reserved.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/10442
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences
Uncontrolled Keywords	carboxylesterase, insecticide resistance, vector, organophosphate, carbamate, mosquito, amplicon, INSECTICIDE RESISTANCE GENES, WORLDWIDE MIGRATION, AMPLIFICATION, MOSQUITOS, QUINQUEFASCIATUS, SEQUENCE, TRANSVECTION, CALIFORNIA
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