Ultrahigh-throughput sample analysis using liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

Krenkel, H., Brown, J., Richardson, K., Hoyes, E., Morris, M. and Cramer, R. ORCID: https://orcid.org/0000-0002-8037-2511 (2022) Ultrahigh-throughput sample analysis using liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry. Analytical Chemistry, 94 (10). pp. 4141-4145. ISSN 0003-2700 doi: 10.1021/acs.analchem.1c05614

Abstract/Summary

Mass spectrometry (MS) allows for automated analysis of complex samples at high resolution without the need for labeling/derivatization. Liquid atmospheric pressure matrix-assisted laser desorption/ionization (LAP-MALDI) enables rapid sample preparation and MS analysis using microtiter-plate formats and high-performing mass spectrometers. We present a step change in high-speed, large-scale MS sample analysis of peptides at 20 samples/s and an enzymatic assay at 40 samples/s, i.e., an order of magnitude faster than current MS platforms. LAP-MALDI requires only low amounts of sample volume (<2 μL), of which only a fraction (<1%) is typically consumed, and allows for multiplexing and high-speed MS/MS analysis, demonstrated at ∼10 samples/s. Its high ion signal stability and similarity to electrospray ionization enables CVs below 10% and the analysis of multiply charged peptide ions at these extreme speeds. LAP-MALDI MS fulfills the speed requirements for large-scale population diagnostics and compound screening with the potential of analyzing >1 million samples per day.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/104028
Identification Number/DOI	10.1021/acs.analchem.1c05614
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Publisher	American Chemical Society
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