Genome-wide association study dissects the genetic control of plant height and branch number in response to low-phosphorus stress in Brassica napus

Liu, H., Wang, J., Zhang, B., Yang, X., Hammond, J. P. ORCID: https://orcid.org/0000-0002-6241-3551, Ding, G., Wang, S., Cai, H., Wang, C., Xu, F. ORCID: https://orcid.org/0000-0003-3564-1644 and Shi, L. ORCID: https://orcid.org/0000-0002-5312-8521 (2021) Genome-wide association study dissects the genetic control of plant height and branch number in response to low-phosphorus stress in Brassica napus. Annals of Botany, 128 (7). ISSN 1095-8290 doi: 10.1093/aob/mcab115

Abstract/Summary

Abstract Background and Aims Oilseed rape (Brassica napus) is one of the most important oil crops worldwide. Phosphorus (P) deficiency severely decreases the plant height and branch number of B. napus. However, the genetic bases controlling plant height and branch number in B. napus under P deficiency remain largely unknown. This study aims to mine candidate genes for plant height and branch number by genome-wide association study (GWAS) and determine low-P-tolerance haplotypes. Methods An association panel of B. napus was grown in the field with a low P supply (P, 0 kg ha−1) and a sufficient P supply (P, 40 kg ha−1) across 2 years and plant height and branch number were investigated. More than five million single-nucleotide polymorphisms (SNPs) were used to conduct GWAS of plant height and branch number at two contrasting P supplies. Key Results A total of 2127 SNPs were strongly associated (P < 6·25 × 10−07) with plant height and branch number at two P supplies. There was significant correlation between phenotypic variation and the number of favourable alleles of associated loci on chromosomes A10 (chrA10_821671) and C08 (chrC08_27999846), which will contribute to breeding improvement by aggregating these SNPs. BnaA10g09290D and BnaC08g26640D were identified to be associated with chrA10_821671 and chrC08_27999846, respectively. Candidate gene association analysis and haplotype analysis showed that the inbred lines carrying ATT at BnaA10g09290Hap1 and AAT at BnaC08g26640Hap1 had greater plant height than lines carrying other haplotype alleles at low P supply. Conclusion Our results demonstrate the power of GWAS in identifying genes of interest in B. napus and provided insights into the genetic basis of plant height and branch number at low P supply in B. napus. Candidate genes and favourable haplotypes may facilitate marker-based breeding efforts aimed at improving P use efficiency in B. napus.

Additional Information	** Article version: AM ** From Crossref journal articles via Jisc Publications Router ** Journal IDs: pissn 0305-7364; eissn 1095-8290 ** History: issued 07-09-2021; published_online 07-09-2021 ** License for this article: starting on 07-09-2021, , https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/101031
Identification Number/DOI	10.1093/aob/mcab115
Divisions	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
Uncontrolled Keywords	Plant Science
Additional Information	** Article version: AM ** From Crossref journal articles via Jisc Publications Router ** Journal IDs: pissn 0305-7364; eissn 1095-8290 ** History: issued 07-09-2021; published_online 07-09-2021 ** License for this article: starting on 07-09-2021, , https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
Publisher	Oxford University Press (OUP)
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