A simple and rapid method for preserving RNA of aquatic invertebrates for ecotoxicogenomics

Heckmann, L. H., Bouetard, A., Hill, C. J., Sibly, R. M. ORCID: https://orcid.org/0000-0001-6828-3543 and Callaghan, A. ORCID: https://orcid.org/0000-0002-2731-3352 (2007) A simple and rapid method for preserving RNA of aquatic invertebrates for ecotoxicogenomics. Ecotoxicology, 16 (6). pp. 445-447. ISSN 0963-9292 doi: 10.1007/s10646-007-0148-y

Abstract/Summary

Here we describe a novel, inexpensive and simple method for preserving RNA that reduces handling stress in aquatic invertebrates following ecotoxicogenomic experimentation. The application of the method is based on transcriptomic experiments conducted on Daphnia magna, but may easily be applied on a range of other aquatic organisms of a particular size with e.g. amphipod Gammarus pulex representing an upper size limit. We explain in detail how to apply this new method, named the "Cylindrical Sieve (CS) system", and highlight its advantages and disadvantages.

Item Type	Article
URI	https://reading-clone.eprints-hosting.org/id/eprint/10008
Item Type	Article
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences
Uncontrolled Keywords	transcriptomics, handling stress, animal welfare, crustacean, Daphnia, magna, GENE-EXPRESSION, ECOTOXICOLOGY
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